NASC

NASC ID: N850123

Name: WiscDsLox264C10

ABRC stock number: CS850123

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox264C10.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G54400.1-UTR3 and AT5G54410.1 (chr 5 pos 22093891 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797188.

Wiscseq_DsLox264C10.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT3G52230.1 (chr 3 pos 19372608 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG797185.

Wiscseq_DsLox264C10.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G32050.1 (chr 4 pos 15499236 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797187.

Wiscseq_DsLox264C10.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G15960.1-P355 and AT4G15970.1-P355 (chr 4 pos 9048356 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797186.

Wiscseq_DsLox264C10.4 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G61997.1 (chr 5 pos 24908291 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797189.

Donation Date: 2006-09-04

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox264C10

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium